Find out how CRISPR-mediated gene activation (CRISPRa) and repression (CRISPRi) works and why you should consider using them in addition to your CRISPR knockouts.
The post Why You Should Consider Adding CRISPRa and CRISPRi to Your Toolbox appeared first on Bitesize Bio.
Stand up for science by fighting the spread of misinformation with our how-to guide.
The post Stand up for Science: How to Detect and Fight Misinformation appeared first on Bitesize Bio.
The threat of pandemic viruses can be mitigated by developing diagnostic tests that are rapidly implementable and field deployable. Two CRISPR-based strategies, DETECTR and SHERLOCK, have been developed recently that not only meet these criteria but offer unprecedented multiplexing capabilities. This article discusses the theoretical basis of proposed and currently available viral diagnostics for educational…
The post How CRISPR Can Be Used to Detect Emerging Viral Pathogens appeared first on Bitesize Bio.
Fret no more over fuzzy bands! We uncover how gradient gels can give you better results, and maximize your precious samples when performing SDS-PAGE
The post A Guide to Gradient Gels: The Why’s and How’s appeared first on Bitesize Bio.
T cells can be tricky to engineer with CRISPR. Find out the key considerations when editing these cells and how you can overcome any associated challenges.
The post A CRISPR Approach to Immuno-Oncology appeared first on Bitesize Bio.
From bad weather to rail strikes or global pandemics, there are several reasons you may find yourself working from home. After writing up any outstanding grant proposals or papers, reviewing the literature, and perusing the endless field of coronavirus news updates, what’s a scientist to do? Brush up on your non-lab skills, of course! We…
The post Online Learning Resources for Scientists Working From Home appeared first on Bitesize Bio.
Find out how modified variants of CRISPR nucleases provide gene editing with reduced off-target effects and can even control gene expression without altering the DNA sequence.
The post How to Understand CRISPR Formats and Their Applications appeared first on Bitesize Bio.
Discover how to validate the success of your CRISPR gene editing experiment from determining successful delivery of CRISPR reagents into your cells to the confirmation of desired genetic and phenotype changes.
The post How to Validate a CRISPR Experiment appeared first on Bitesize Bio.
Are you facing working from home? Find out how to prepare and stay productive.
The post How to Prepare for Working From Home as a Research Scientist appeared first on Bitesize Bio.
Discover how to get started with CRISPR gene editing in your experiments with our key considerations.
The post CRISPR Gene Editing: Considerations and Getting Started appeared first on Bitesize Bio.
DOIs got you confused? Find out what they are and how to use them.
The post What is a DOI and Why Should You Care? appeared first on Bitesize Bio.
Cell in culture need serum to survive and grow, right? Or is it? Can your cells survive without serum and why should you try?
The post Going Serum-Free: The How and Why of Removing Serum From Your Media appeared first on Bitesize Bio.
3' mRNA-seq is a NGS method to profile gene expression that is sensitive and straightforward but won’t blow your budget.
The post 3′ mRNA-Seq is Transforming Gene Expression Analysis appeared first on Bitesize Bio.
We are all familiar with bacterial cell lines as a means for protein expression and purification. But can you do the same with eukaryotic cell lines? Read on to learn some helpful tips and considerations when needing to get your hands on some eukaryotic protein.
The post How to Use a Eukaryotic System for Protein Expression & Purification appeared first on Bitesize Bio.
While DNA and RNA extraction is a pretty common technique, it isn't always the easiest. Read on to learn some top tips on how to successfully extract nucleic acids from even the toughest samples, like Arabidopsis seeds.
The post Isolating Nucleic Acids From Arabidopsis Seeds (And Other Tough Seeds) appeared first on Bitesize Bio.
PubMed has had a makeover. Find out where your favorite features have moved to and what's changed in this shiny new version.
The post Out With the Old: New PubMed Search Tips appeared first on Bitesize Bio.
Have you got a plasmid that others are begging to use? Perhaps your cell line is in high demand? We'll show how using a repository can take the hassle out of sharing your reagents.
The post Share Your Samples at a Reagent Repository appeared first on Bitesize Bio.
Mitochondrial DNA isolation can be time-consuming and laborious. Find out how to minimise the time needed for its extraction, while ensuring fantastic results.
The post How to Isolate DNA from Mitochondria: An Energetic Guide appeared first on Bitesize Bio.
There is an overwhelming selection of cell lines available, make sure you pick the right one for your work using our tips.
The post The Do’s and Dont’s of Choosing a Cell Line appeared first on Bitesize Bio.
Working from home but don't have a garage lab? We've got 10 ideas to keep you productive while you're working away from the bench.
The post 10 Ideas for Researchers Working from Home appeared first on Bitesize Bio.
