Wednesday, 17 April 2019

The EMSA – Teaching an Old Dog New Tricks

Probing Nucleic Acid-Protein Interactions with EMSA The electrophoretic mobility shift assay (EMSA) is a powerful technique for detecting specific-binding of nucleic acid-protein complexes. Over the past 30 years, EMSA has been the “go to assay” to investigate the qualitative interactions between nucleic acids (DNA or RNA) and nucleic-acid binding proteins. Through the use of radio-labeled…

The post The EMSA – Teaching an Old Dog New Tricks appeared first on Bitesize Bio.

Tuesday, 16 April 2019

Mastering the Art of Isolating Pure Alveolar Epithelial Cells

Alveolar epithelial cells (AECs) are one of the major types of lung cells that can be used to analyze the response of lung epithelia to external agents. AECs from mouse lungs can thus be utilized as an in vitro model of diseases. AECs are indispensable for studying lung development, injury, and repair. People working on…

The post Mastering the Art of Isolating Pure Alveolar Epithelial Cells appeared first on Bitesize Bio.

Thursday, 11 April 2019

Emerging Model Microorganisms Take to the Stage

Estimates indicate that there may be up to 2 billion living species of organisms, each with conserved and unique biological mechanisms that are vital for survival. How do scientists understand them all? Enter model organisms. Model organisms, as the name implies, are living things which are used as representative models for understanding other organisms. They…

The post Emerging Model Microorganisms Take to the Stage appeared first on Bitesize Bio.

Monday, 8 April 2019

Isolating Bacterial RNA from Blood

For many decades, the only way to detect sepsis – bacterial growth in blood – was isolating the bacteria and growing bacterial colonies on a special medium. This was done by first spinning down the blood, which brought the blood cells and bacteria into the pellet. The pellet was spread on a blood agar plate…

The post Isolating Bacterial RNA from Blood appeared first on Bitesize Bio.

Thursday, 4 April 2019

Ultramicrotomy for Electron Microscopy

Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…

The post Ultramicrotomy for Electron Microscopy appeared first on Bitesize Bio.

Wednesday, 3 April 2019

Microscope Finder Slides: Their History, Development, and Use

Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…

The post Microscope Finder Slides: Their History, Development, and Use appeared first on Bitesize Bio.

Wednesday, 27 March 2019

How (and Why) to Label Nucleic Acids

Have you ever wished you could snag individual strands of DNA or RNA with a lasso? Or look at them one by one, figuring out exactly where they are or what they are doing? Fortunately, there are techniques that exist to label nucleic acids for their visualization and purification! Nucleic acids can be labeled at…

The post How (and Why) to Label Nucleic Acids appeared first on Bitesize Bio.