Wednesday, 15 November 2017

Quantifying Allele-Specific Gene Expression Using PCR-Based Methods

Quantifying Allele-Specific Gene Expression Using PCR-Based Methods 

Allele-specific expression can occur for various biological reasons, such as gene imprinting, or differential transcription caused by mutations, or single nucleotide polymorphisms (SNPs), or epigenetic alterations. Traditional end-point RT-PCR or qRT-PCR-based methods only detect overall levels of mRNA expression from a given gene rather than mRNA transcripts originating from individuals. If your project requires more…

The post Quantifying Allele-Specific Gene Expression Using PCR-Based Methods appeared first on Bitesize Bio.

Tuesday, 14 November 2017

iPOND, Part 1: Fishing for Proteins with DNA as Bait

iPOND, Part 1: Fishing for Proteins with DNA as Bait 

No, iPOND is not a sleek electronic fishing device from Apple. However, if you thought about fishing, well, you’re not far off the mark. If you find yourself wondering which proteins are present at DNA during or after replication, iPOND is an elegant technique to help you find out. In this article, I will explain…

The post iPOND, Part 1: Fishing for Proteins with DNA as Bait appeared first on Bitesize Bio.

Monday, 6 November 2017

Guide to Making and Storing Competent Yeast Cells

Guide to Making and Storing Competent Yeast Cells 

Yeasts, such as Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris, are routinely used in biology research labs around the world. Yeasts are easy-to-culture, unicellular eukaryotes, and make excellent model organisms because of the similarity of their genes and proteins with those of their mammalian counterparts. Yeast cells are used to study gene function, protein interactions,…

The post Guide to Making and Storing Competent Yeast Cells appeared first on Bitesize Bio.

How to Get Over Impostor Syndrome as a New Graduate Student

How to Get Over Impostor Syndrome as a New Graduate Student 

You made it. You got into the grad school of your dreams! You worked hard, you spent hours working on your application, bravely navigated your way through the interview and you now are here. So, why do you feel like maybe you shouldn’t be? Why You Might Suffer From Impostor Syndrome The dreaded impostor syndrome:…

The post How to Get Over Impostor Syndrome as a New Graduate Student appeared first on Bitesize Bio.

From Cells to Scope: Chamber Slide Immunochemistry

From Cells to Scope: Chamber Slide Immunochemistry 

Immunolabeling is the tried-and-true immunochemistry method of getting the stain you want onto the molecular target you want. Whether that target is contained within a large region of tissue (immunohistochemistry) or inside a single cell (immunocytochemistry), the ability to accurately label large numbers of samples will simplify your workflow and help you to achieve excellent…

The post From Cells to Scope: Chamber Slide Immunochemistry appeared first on Bitesize Bio.

Sunday, 29 October 2017

Bacterial Transformation Troubleshooting for Beginners

Bacterial Transformation Troubleshooting for Beginners 

The first time I did a transformation was when I worked with site directed mutagenesis. I cloned a protein sequence into the p15TVL vector, created my mutants (but that’s another story), and was finally ready for the next step: transformation and expression of my desired protein. Little did I know that my enthusiasm would fall…

The post Bacterial Transformation Troubleshooting for Beginners appeared first on Bitesize Bio.

Thursday, 26 October 2017

An Introduction to In-Gel Zymography

An Introduction to In-Gel Zymography 

Enzymes are special among proteins. It is not enough to detect them. You need to know their activity level. If you have devoted a substantial part of your research to studying proteases, like I did, you’ll know how crucial it is to choose an appropriate enzyme assay. There’s a heap of lab techniques out there…

The post An Introduction to In-Gel Zymography appeared first on Bitesize Bio.