Tuesday, 23 May 2017

The 3 Most Common Flow Cytometry Fallacies

The 3 Most Common Flow Cytometry Fallacies 

Flow cytometry is fast evolving from a method only revered by immunologists, to one used by nearly every biological specialty. It’s pretty much my favorite tool. Unfortunately, as with most lab techniques, much of flow cytometry is taught on the job without a lot of standards. And too often bad habits are passed along like…

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Solvent Extraction of Medicinal Compounds from Plants

Solvent Extraction of Medicinal Compounds from Plants 

Extraction for pharmaceutical purposes involves the separation of medicinally active portions of plant or animal tissues from inactive or inert components.  Standardized extraction procedures for crude drugs from plants involve the use of a solvent known as the menstruum to separate the drug from the inactive portion of the plant material. The obtained extract may…

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Sunday, 21 May 2017

Don’t Let Bubbles Burst Your Experimental Excitement

Don’t Let Bubbles Burst Your Experimental Excitement 

Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…

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Saturday, 20 May 2017

Simple Tips for Model Organism-Based Work

Simple Tips for Model Organism-Based Work 

Simple Tips for Model Organism-Based Work The mouse is the favored model organism for life science researchers so much so that mice account for about 95% of all lab animals used in research. The striking similarities between the human and mouse genomes, ease of genetic manipulation and the uniformity achieved through inbred mating makes them…

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Friday, 19 May 2017

Explained: Sensor Chips for Surface Plasmon Resonance and Other Applications

Explained: Sensor Chips for Surface Plasmon Resonance and Other Applications 

Biosensor chip selection is a critical step in planning and running a surface plasmon resonance (SPR) experiment. Chip selection depends on the ligand or target that needs to be immobilized on the sensor chip, the analyte that is flowed over the target to study the binding, and the purpose of the biosensor assay (i.e., determination…

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Go Fishing for Your Favorite Protein with Immunoprecipitation!

Go Fishing for Your Favorite Protein with Immunoprecipitation! 

You have your favorite protein in mind and are ready to set up some exciting experiments to show what it does and how it does it, when it is active, what other proteins it modifies, and how it affects your cells. There is one slight problem. You need to fish your favorite protein out from the…

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Find Out if Your Proteins Do a Slow Dance Together in the Dark

Find Out if Your Proteins Do a Slow Dance Together in the Dark 

Do you wonder if your favorite protein is entwined in a delicate dance with another protein? Do you wish that you could shine a spotlight on your protein to determine its dance partner? Well good news! While there is no such spotlight, you CAN use co-immunoprecipitation (Co-IP) to find your protein’s dance partner. You can…

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