Thursday, 14 December 2017

A Beginner’s Guide to Exosome Isolation

A Beginner’s Guide to Exosome Isolation 

For all of you who have never heard of exosomes: You are missing out on a whole new paradigm in cell-to-cell communication. Exosomes are tiny extracellular vesicles that arise from fusion of the plasma membrane with specific endosomal compartments called multivesicular bodies. Most cells types make exosomes, and release them in order to communicate with…

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Tuesday, 12 December 2017

Modeling – in the World of Proteins

Modeling – in the World of Proteins 

Computational protein structure prediction provides three-dimensional structures of proteins that are predicted by in-silico techniques. Such protein modeling relies on principles from known protein structures obtained via x-Ray crystallography, NMR Spectroscopy, as well as from physical energy functions.  There are three main methods of modeling: The first and favorite method is Homology Modeling,1-2 Followed by the…

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What to Do If You Want to Become a Freelance Writer

What to Do If You Want to Become a Freelance Writer 

A lot of people today are drawn to freelancing and want to become a freelance writer. There are great benefits when you work from home (like doing work in your pajamas) or wherever you feel most comfortable. The appeal is even greater when you count in that you make your own hours and you are…

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Friday, 8 December 2017

The Amazing World of Biofilms

The Amazing World of Biofilms 

What do water pipe slime, dental plaque, and persistent contact lens case contamination have in common? All are the result of biofilms! Biofilms are aggregates of microbes that adhere to surfaces using secreted matrices. Although relatively under explored, this fascinating phenomenon plays a critical role in some of the biggest challenges currently facing medicine, ranging…

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Color Transmission Electron Microscopy

Color Transmission Electron Microscopy 

There are two types of electron microscopy—transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM creates fascinating 2D images by bouncing electrons off the surface of the sample. I highly recommend searching for SEM samples on Google images. While SEM images are aesthetically amazing, the TEM images bring us inside the world of the…

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Thursday, 7 December 2017

The qRT-rtPCR Control You Should Be Doing, But Probably Aren’t

The qRT-rtPCR Control You Should Be Doing, But Probably Aren’t 

Every man, woman, and dog is doing quantitative real time reverse transcriptase PCR (qRT-rtPCR) these days. It’s a great method to measure your favorite transcript’s expression levels. One of the big plusses (like the Swiss flag!) of quantitative PCR in general is its high sensitivity. In principle, it can detect and quantify one molecule of…

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Why Isn’t My Culture Growing? The S-Curve Explained

Why Isn’t My Culture Growing? The S-Curve Explained 

Whether you work with human cell lines or microbes, their growth is governed by the same principles. I invite you to learn about something that lies at the base of any work with cell culture — whether cells have circular or linear chromosomes—the S-curve of the population growth. The length of each phase depends on…

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