Thursday, 24 May 2018

Nine Tips for Clinicians Starting a Scientific Career

There are many examples of the impact of physician-scientists on translational research. Dr Barry Marshall swallowed a steaming culture of Helicobacter pylori which eventually resulted in antibiotics curing peptic ulcer disease. However, the process of training these individuals is as effortless as training fish to ride bicycles. Our journeys into the laboratory have been equally…

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An Introduction to Cardiac Optical Mapping

What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart.  In pathological conditions, these electrical impulses can become disordered and lead…

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Wednesday, 23 May 2018

New-ISH on the Block: Introduction to RNAscope®

When sensitive detection of RNA is required, many scientists turn to qPCR as it is a versatile technique that can detect many different types of RNAs from mRNA, non-coding RNA, to microRNA. However, if you also require spatial information, like which cells are producing your RNA, the technology of choice has historically been in situ…

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Tuesday, 22 May 2018

Generating High-Quality Genome Assemblies from Metagenomic Sequencing

The decreasing costs in genomic sequencing over the past decade have inspired researchers to apply shotgun next-generation sequencing to entire microbial communities. While the reads generated typically cannot be assembled cleanly into individual genomes, there is often enough information produced to identify most microbes present in the population. However, this approach lacks sufficient resolution to…

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Monday, 21 May 2018

RNA Isolation from Drosophila – Don’t Let the Cuticle Scare You!

Isolating RNA from either Drosophila larvae or adult heads can seem a bit daunting, primarily due to the presence of the cuticle. The cuticle is a protective exoskeleton comprised of insoluble collagens, cuticlins, glycoproteins, and lipids. While it may take some force to remove the cuticle, you can do this easily and without compromising your…

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Immuno-PCR: A Highly Sensitive Method of Immunodetection

Researchers have relied on immunodetection techniques such as Western blotting, flow cytometry and Enzyme-Linked Immunosorbent Assay (ELISA) for years, but immuno-PCR is a relatively new method. By merging an ELISA with the Polymerase Chain Reaction (PCR), immuno-PCR provides extremely high levels of assay sensitivity. ELISA An ELISA is an assay in which a molecule is…

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Friday, 18 May 2018

Fishing for Kinases with Multiplex Inhibitor Bead Assays

There is something about kinases that resemble ghosts. Their effects reveal their presence, but they can be difficult to catch. With a low abundance of hundreds or even tens of molecules per cell, they are difficult to detect using conventional methods such as Western blotting or mass spectrometry (MS). However, you will need to detect…

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