Thursday 18 August 2016

Methods for Relative Quantification of qPCR Data. Yes, There is More Than One.

Methods for Relative Quantification of qPCR Data. Yes, There is More Than One. 

As all of you probably know, methods for calculating relative gene expression from qPCR data include: a) double delta Ct (ΔΔCt) and b) that one other method. Chances are you’ve probably gotten beyond the ΔΔCt method, but you should be prepared in case you face primer sets of different amplification efficiencies. Both methods require the […]

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