Sunday, 11 December 2016

How to Dephsophorylate DNA Using Calf Intestinal Phosphatase

How to Dephsophorylate DNA Using Calf Intestinal Phosphatase 

When you dephosphorylate a vector for cloning, you remove the 5′ phosphate groups required by DNA ligase to join the phosphodiester DNA backbone together. This prevents your vector from ligating back to itself during the ligation step and decreases your background. Various alkaline phosphatases exist, including calf intestinal phosphatase (CIP), Shrimp and Antarctic phosphatases. The […]

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